Translational_Unit

Part:BBa_K3994013:Design

Designed by: Nixue Song   Group: iGEM21_NOFLS_YZ   (2021-10-12)


PhrpL_amilGFP_IL10_PJ23104_ttrS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 591
    Illegal EcoRI site found at 3861
    Illegal PstI site found at 537
    Illegal PstI site found at 744
    Illegal PstI site found at 771
    Illegal PstI site found at 1068
    Illegal PstI site found at 3309
    Illegal PstI site found at 3828
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 591
    Illegal EcoRI site found at 3861
    Illegal NheI site found at 2163
    Illegal NheI site found at 2186
    Illegal PstI site found at 537
    Illegal PstI site found at 744
    Illegal PstI site found at 771
    Illegal PstI site found at 1068
    Illegal PstI site found at 3309
    Illegal PstI site found at 3828
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 591
    Illegal EcoRI site found at 3861
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 591
    Illegal EcoRI site found at 3861
    Illegal PstI site found at 537
    Illegal PstI site found at 744
    Illegal PstI site found at 771
    Illegal PstI site found at 1068
    Illegal PstI site found at 3309
    Illegal PstI site found at 3828
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 591
    Illegal EcoRI site found at 3861
    Illegal PstI site found at 537
    Illegal PstI site found at 744
    Illegal PstI site found at 771
    Illegal PstI site found at 1068
    Illegal PstI site found at 3309
    Illegal PstI site found at 3828
    Illegal AgeI site found at 336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

No

References